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human irs1-shrna-1  (Addgene inc)


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    Addgene inc human irs1-shrna-1
    A Western blot analysis of PTEN, p-Akt s473, t-Akt, <t>p-IRS1</t> Y612, and t-IRS1 expression in BGC803 and MKN45 cells transfected with PTEN siRNAs. B Western blot analysis of IRS1, p-Akt t308, p-Akt s473, t-Akt, and PTEN expression in BGC803 and MKN45 cells infected with lentivirus expressing IRS1-specific shRNA or scramble shRNA. Cell proliferation ability detection of BGC803 and MKN45 cells after IRS1 knockdown by ( C ) CCK-8 assay, ( D ) plate clone formation assay, ( E ) soft agar clone formation assay, and ( F ) EdU assay. *** p < 0.001, ** p < 0.01, * p < 0.05.
    Human Irs1 Shrna 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human irs1-shrna-1/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    human irs1-shrna-1 - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer"

    Article Title: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

    Journal: Oncogene

    doi: 10.1038/s41388-023-02619-4

    A Western blot analysis of PTEN, p-Akt s473, t-Akt, p-IRS1 Y612, and t-IRS1 expression in BGC803 and MKN45 cells transfected with PTEN siRNAs. B Western blot analysis of IRS1, p-Akt t308, p-Akt s473, t-Akt, and PTEN expression in BGC803 and MKN45 cells infected with lentivirus expressing IRS1-specific shRNA or scramble shRNA. Cell proliferation ability detection of BGC803 and MKN45 cells after IRS1 knockdown by ( C ) CCK-8 assay, ( D ) plate clone formation assay, ( E ) soft agar clone formation assay, and ( F ) EdU assay. *** p < 0.001, ** p < 0.01, * p < 0.05.
    Figure Legend Snippet: A Western blot analysis of PTEN, p-Akt s473, t-Akt, p-IRS1 Y612, and t-IRS1 expression in BGC803 and MKN45 cells transfected with PTEN siRNAs. B Western blot analysis of IRS1, p-Akt t308, p-Akt s473, t-Akt, and PTEN expression in BGC803 and MKN45 cells infected with lentivirus expressing IRS1-specific shRNA or scramble shRNA. Cell proliferation ability detection of BGC803 and MKN45 cells after IRS1 knockdown by ( C ) CCK-8 assay, ( D ) plate clone formation assay, ( E ) soft agar clone formation assay, and ( F ) EdU assay. *** p < 0.001, ** p < 0.01, * p < 0.05.

    Techniques Used: Western Blot, Expressing, Transfection, Infection, shRNA, CCK-8 Assay, Tube Formation Assay, EdU Assay

    A Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after Dox-induced silencing of NEDD4. NEDD4-con, scramble shRNA; NEDD4-sh1 and sh2, NEDD4 shRNA constructs 1 and 2. B Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after NEDD4 overexpression. Cell proliferation ability detection of BGC803 and MKN45 cells after NEDD4 knockdown and overexpression by ( C , D ) CCK-8 assay, ( E ) plate clone formation assay, ( F ) soft agar clone formation assay, and ( G , H ) EdU assay. I Western blot analysis of p-Akt s473 and t-Akt protein levels in BGC803 and MKN45 cells after treatment with Heclin (10 μM) for 48 h. J Cell viability detection of cells treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.
    Figure Legend Snippet: A Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after Dox-induced silencing of NEDD4. NEDD4-con, scramble shRNA; NEDD4-sh1 and sh2, NEDD4 shRNA constructs 1 and 2. B Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after NEDD4 overexpression. Cell proliferation ability detection of BGC803 and MKN45 cells after NEDD4 knockdown and overexpression by ( C , D ) CCK-8 assay, ( E ) plate clone formation assay, ( F ) soft agar clone formation assay, and ( G , H ) EdU assay. I Western blot analysis of p-Akt s473 and t-Akt protein levels in BGC803 and MKN45 cells after treatment with Heclin (10 μM) for 48 h. J Cell viability detection of cells treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.

    Techniques Used: Western Blot, shRNA, Construct, Over Expression, CCK-8 Assay, Tube Formation Assay, EdU Assay

    A–C Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in AGS cells after silencing of IRS1 and NEDD4 and overexpressing NEDD4. Cell proliferation ability detection of AGS cells after IRS1 and NEDD4 knockdown and NEDD4 overexpression, respectively, by ( D–F ) CCK-8 assay, ( G , H ) plate clone formation assay, and ( I–K ) EdU assay. L Western blot analysis of p-Akt s473 and t-Akt in AGS cells after treatment of Heclin(10 μM) for 48 h. M Cell viability detection of AGS treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.
    Figure Legend Snippet: A–C Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in AGS cells after silencing of IRS1 and NEDD4 and overexpressing NEDD4. Cell proliferation ability detection of AGS cells after IRS1 and NEDD4 knockdown and NEDD4 overexpression, respectively, by ( D–F ) CCK-8 assay, ( G , H ) plate clone formation assay, and ( I–K ) EdU assay. L Western blot analysis of p-Akt s473 and t-Akt in AGS cells after treatment of Heclin(10 μM) for 48 h. M Cell viability detection of AGS treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.

    Techniques Used: Western Blot, Over Expression, CCK-8 Assay, Tube Formation Assay, EdU Assay

    A , B Western blot analysis of NEDD4, PTEN, p-Akt s473, t-Akt, p-IRS Y612, and t-IRS1 protein level in BGC803 and MKN45 cells infected with lentivirus expressing scramble shRNA (control), shNEDD4-1 and shNEDD4-2 combined with PTEN siRNA. C , D CCK-8 assay and ( E , F ) EdU assay showing proliferation ability of the indicated cells. *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.
    Figure Legend Snippet: A , B Western blot analysis of NEDD4, PTEN, p-Akt s473, t-Akt, p-IRS Y612, and t-IRS1 protein level in BGC803 and MKN45 cells infected with lentivirus expressing scramble shRNA (control), shNEDD4-1 and shNEDD4-2 combined with PTEN siRNA. C , D CCK-8 assay and ( E , F ) EdU assay showing proliferation ability of the indicated cells. *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.

    Techniques Used: Western Blot, Infection, Expressing, shRNA, CCK-8 Assay, EdU Assay

    Correlations between IRS1 expression and clinical characteristics in patients with GC.
    Figure Legend Snippet: Correlations between IRS1 expression and clinical characteristics in patients with GC.

    Techniques Used: Expressing

    A Representative images of IGF1R, NEDD4, and p-Akt s473 expression in adjacent non-tumor tissues and primary GC tissues detected by IHC staining. B IHC scores of IGF1R, NEDD4, and p-Akt S473 expressions in adjacent non-tumor tissues and primary GC tissues. C Analysis of IGF1 and NEDD4 expressions in normal tissue and GC tissues from Gastric datasets (GSE3468354 and GSE27342, N, normal gastric mucosa, n = 80; T, gastric tumor, n = 80). D Analysis of IGF1R expression in GC stratified by N-cadherin expression and normal tissues, NEDD4 expression in GC stratified by N-cadherin expression and normal tissues, and NEDD4 expression in GC stratified by IGF1 expression and normal tissues in TCGA database. E Protein expression correlation of IGF1R, NEDD4, and p-Akt s473 in GC tissues. F Kaplan–Meier plots of OS among GC patients with different expressions of IGF1, IGF1R, IRS1, and NEDD4. G Overall survival rate of STAD from the TCGA database was analyzed according to the mRNA levels of IGF1 and NEDD4, NEDD4 and N-cadherin. IGF1-High/NEDD4-High ( n = 103); IGF1-High/NEDD4-Low ( n = 82); IGF1-Low/NEDD4-High ( n = 82); IGF1-Low/NEDD4-Low ( n = 103). NEDD4-High/N-cadherin-High ( n = 111); NEDD4-High/N-cadherin-Low ( n = 74); NEDD4-Low/N-cadherin-High ( n = 74); NEDD4-Low/N-cadherin-Low ( n = 111). *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.
    Figure Legend Snippet: A Representative images of IGF1R, NEDD4, and p-Akt s473 expression in adjacent non-tumor tissues and primary GC tissues detected by IHC staining. B IHC scores of IGF1R, NEDD4, and p-Akt S473 expressions in adjacent non-tumor tissues and primary GC tissues. C Analysis of IGF1 and NEDD4 expressions in normal tissue and GC tissues from Gastric datasets (GSE3468354 and GSE27342, N, normal gastric mucosa, n = 80; T, gastric tumor, n = 80). D Analysis of IGF1R expression in GC stratified by N-cadherin expression and normal tissues, NEDD4 expression in GC stratified by N-cadherin expression and normal tissues, and NEDD4 expression in GC stratified by IGF1 expression and normal tissues in TCGA database. E Protein expression correlation of IGF1R, NEDD4, and p-Akt s473 in GC tissues. F Kaplan–Meier plots of OS among GC patients with different expressions of IGF1, IGF1R, IRS1, and NEDD4. G Overall survival rate of STAD from the TCGA database was analyzed according to the mRNA levels of IGF1 and NEDD4, NEDD4 and N-cadherin. IGF1-High/NEDD4-High ( n = 103); IGF1-High/NEDD4-Low ( n = 82); IGF1-Low/NEDD4-High ( n = 82); IGF1-Low/NEDD4-Low ( n = 103). NEDD4-High/N-cadherin-High ( n = 111); NEDD4-High/N-cadherin-Low ( n = 74); NEDD4-Low/N-cadherin-High ( n = 74); NEDD4-Low/N-cadherin-Low ( n = 111). *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.

    Techniques Used: Expressing, Immunohistochemistry



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    human irs1-shrna-1  (Addgene inc)
    90
    Addgene inc human irs1-shrna-1
    A Western blot analysis of PTEN, p-Akt s473, t-Akt, <t>p-IRS1</t> Y612, and t-IRS1 expression in BGC803 and MKN45 cells transfected with PTEN siRNAs. B Western blot analysis of IRS1, p-Akt t308, p-Akt s473, t-Akt, and PTEN expression in BGC803 and MKN45 cells infected with lentivirus expressing IRS1-specific shRNA or scramble shRNA. Cell proliferation ability detection of BGC803 and MKN45 cells after IRS1 knockdown by ( C ) CCK-8 assay, ( D ) plate clone formation assay, ( E ) soft agar clone formation assay, and ( F ) EdU assay. *** p < 0.001, ** p < 0.01, * p < 0.05.
    Human Irs1 Shrna 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human irs1-shrna-1/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    human irs1-shrna-1 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A Western blot analysis of PTEN, p-Akt s473, t-Akt, p-IRS1 Y612, and t-IRS1 expression in BGC803 and MKN45 cells transfected with PTEN siRNAs. B Western blot analysis of IRS1, p-Akt t308, p-Akt s473, t-Akt, and PTEN expression in BGC803 and MKN45 cells infected with lentivirus expressing IRS1-specific shRNA or scramble shRNA. Cell proliferation ability detection of BGC803 and MKN45 cells after IRS1 knockdown by ( C ) CCK-8 assay, ( D ) plate clone formation assay, ( E ) soft agar clone formation assay, and ( F ) EdU assay. *** p < 0.001, ** p < 0.01, * p < 0.05.

    Journal: Oncogene

    Article Title: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

    doi: 10.1038/s41388-023-02619-4

    Figure Lengend Snippet: A Western blot analysis of PTEN, p-Akt s473, t-Akt, p-IRS1 Y612, and t-IRS1 expression in BGC803 and MKN45 cells transfected with PTEN siRNAs. B Western blot analysis of IRS1, p-Akt t308, p-Akt s473, t-Akt, and PTEN expression in BGC803 and MKN45 cells infected with lentivirus expressing IRS1-specific shRNA or scramble shRNA. Cell proliferation ability detection of BGC803 and MKN45 cells after IRS1 knockdown by ( C ) CCK-8 assay, ( D ) plate clone formation assay, ( E ) soft agar clone formation assay, and ( F ) EdU assay. *** p < 0.001, ** p < 0.01, * p < 0.05.

    Article Snippet: The human IRS1-shRNA-1, human IRS1-shRNA-2, human NEDD4-shRNA-1, and human NEDD4-shRNA-2 were contracted into pLKO.1-TRC cloning vector (Addgene, Cambridge, MA, USA; 10878).

    Techniques: Western Blot, Expressing, Transfection, Infection, shRNA, CCK-8 Assay, Tube Formation Assay, EdU Assay

    A Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after Dox-induced silencing of NEDD4. NEDD4-con, scramble shRNA; NEDD4-sh1 and sh2, NEDD4 shRNA constructs 1 and 2. B Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after NEDD4 overexpression. Cell proliferation ability detection of BGC803 and MKN45 cells after NEDD4 knockdown and overexpression by ( C , D ) CCK-8 assay, ( E ) plate clone formation assay, ( F ) soft agar clone formation assay, and ( G , H ) EdU assay. I Western blot analysis of p-Akt s473 and t-Akt protein levels in BGC803 and MKN45 cells after treatment with Heclin (10 μM) for 48 h. J Cell viability detection of cells treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.

    Journal: Oncogene

    Article Title: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

    doi: 10.1038/s41388-023-02619-4

    Figure Lengend Snippet: A Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after Dox-induced silencing of NEDD4. NEDD4-con, scramble shRNA; NEDD4-sh1 and sh2, NEDD4 shRNA constructs 1 and 2. B Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in BGC803 and MKN45 cells after NEDD4 overexpression. Cell proliferation ability detection of BGC803 and MKN45 cells after NEDD4 knockdown and overexpression by ( C , D ) CCK-8 assay, ( E ) plate clone formation assay, ( F ) soft agar clone formation assay, and ( G , H ) EdU assay. I Western blot analysis of p-Akt s473 and t-Akt protein levels in BGC803 and MKN45 cells after treatment with Heclin (10 μM) for 48 h. J Cell viability detection of cells treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.

    Article Snippet: The human IRS1-shRNA-1, human IRS1-shRNA-2, human NEDD4-shRNA-1, and human NEDD4-shRNA-2 were contracted into pLKO.1-TRC cloning vector (Addgene, Cambridge, MA, USA; 10878).

    Techniques: Western Blot, shRNA, Construct, Over Expression, CCK-8 Assay, Tube Formation Assay, EdU Assay

    A–C Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in AGS cells after silencing of IRS1 and NEDD4 and overexpressing NEDD4. Cell proliferation ability detection of AGS cells after IRS1 and NEDD4 knockdown and NEDD4 overexpression, respectively, by ( D–F ) CCK-8 assay, ( G , H ) plate clone formation assay, and ( I–K ) EdU assay. L Western blot analysis of p-Akt s473 and t-Akt in AGS cells after treatment of Heclin(10 μM) for 48 h. M Cell viability detection of AGS treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.

    Journal: Oncogene

    Article Title: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

    doi: 10.1038/s41388-023-02619-4

    Figure Lengend Snippet: A–C Western blot analysis of NEDD4, p-Akt s473, t-Akt, p-IRS1 Y612, t-IRS1, and PTEN protein level in AGS cells after silencing of IRS1 and NEDD4 and overexpressing NEDD4. Cell proliferation ability detection of AGS cells after IRS1 and NEDD4 knockdown and NEDD4 overexpression, respectively, by ( D–F ) CCK-8 assay, ( G , H ) plate clone formation assay, and ( I–K ) EdU assay. L Western blot analysis of p-Akt s473 and t-Akt in AGS cells after treatment of Heclin(10 μM) for 48 h. M Cell viability detection of AGS treated with Heclin (10 μM) by CCK-8 assay. *** p < 0.001, ** p < 0.01, * p < 0.05, ns > 0.05.

    Article Snippet: The human IRS1-shRNA-1, human IRS1-shRNA-2, human NEDD4-shRNA-1, and human NEDD4-shRNA-2 were contracted into pLKO.1-TRC cloning vector (Addgene, Cambridge, MA, USA; 10878).

    Techniques: Western Blot, Over Expression, CCK-8 Assay, Tube Formation Assay, EdU Assay

    A , B Western blot analysis of NEDD4, PTEN, p-Akt s473, t-Akt, p-IRS Y612, and t-IRS1 protein level in BGC803 and MKN45 cells infected with lentivirus expressing scramble shRNA (control), shNEDD4-1 and shNEDD4-2 combined with PTEN siRNA. C , D CCK-8 assay and ( E , F ) EdU assay showing proliferation ability of the indicated cells. *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.

    Journal: Oncogene

    Article Title: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

    doi: 10.1038/s41388-023-02619-4

    Figure Lengend Snippet: A , B Western blot analysis of NEDD4, PTEN, p-Akt s473, t-Akt, p-IRS Y612, and t-IRS1 protein level in BGC803 and MKN45 cells infected with lentivirus expressing scramble shRNA (control), shNEDD4-1 and shNEDD4-2 combined with PTEN siRNA. C , D CCK-8 assay and ( E , F ) EdU assay showing proliferation ability of the indicated cells. *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.

    Article Snippet: The human IRS1-shRNA-1, human IRS1-shRNA-2, human NEDD4-shRNA-1, and human NEDD4-shRNA-2 were contracted into pLKO.1-TRC cloning vector (Addgene, Cambridge, MA, USA; 10878).

    Techniques: Western Blot, Infection, Expressing, shRNA, CCK-8 Assay, EdU Assay

    Correlations between IRS1 expression and clinical characteristics in patients with GC.

    Journal: Oncogene

    Article Title: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

    doi: 10.1038/s41388-023-02619-4

    Figure Lengend Snippet: Correlations between IRS1 expression and clinical characteristics in patients with GC.

    Article Snippet: The human IRS1-shRNA-1, human IRS1-shRNA-2, human NEDD4-shRNA-1, and human NEDD4-shRNA-2 were contracted into pLKO.1-TRC cloning vector (Addgene, Cambridge, MA, USA; 10878).

    Techniques: Expressing

    A Representative images of IGF1R, NEDD4, and p-Akt s473 expression in adjacent non-tumor tissues and primary GC tissues detected by IHC staining. B IHC scores of IGF1R, NEDD4, and p-Akt S473 expressions in adjacent non-tumor tissues and primary GC tissues. C Analysis of IGF1 and NEDD4 expressions in normal tissue and GC tissues from Gastric datasets (GSE3468354 and GSE27342, N, normal gastric mucosa, n = 80; T, gastric tumor, n = 80). D Analysis of IGF1R expression in GC stratified by N-cadherin expression and normal tissues, NEDD4 expression in GC stratified by N-cadherin expression and normal tissues, and NEDD4 expression in GC stratified by IGF1 expression and normal tissues in TCGA database. E Protein expression correlation of IGF1R, NEDD4, and p-Akt s473 in GC tissues. F Kaplan–Meier plots of OS among GC patients with different expressions of IGF1, IGF1R, IRS1, and NEDD4. G Overall survival rate of STAD from the TCGA database was analyzed according to the mRNA levels of IGF1 and NEDD4, NEDD4 and N-cadherin. IGF1-High/NEDD4-High ( n = 103); IGF1-High/NEDD4-Low ( n = 82); IGF1-Low/NEDD4-High ( n = 82); IGF1-Low/NEDD4-Low ( n = 103). NEDD4-High/N-cadherin-High ( n = 111); NEDD4-High/N-cadherin-Low ( n = 74); NEDD4-Low/N-cadherin-High ( n = 74); NEDD4-Low/N-cadherin-Low ( n = 111). *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.

    Journal: Oncogene

    Article Title: Targeting the E3 ligase NEDD4 as a novel therapeutic strategy for IGF1 signal pathway-driven gastric cancer

    doi: 10.1038/s41388-023-02619-4

    Figure Lengend Snippet: A Representative images of IGF1R, NEDD4, and p-Akt s473 expression in adjacent non-tumor tissues and primary GC tissues detected by IHC staining. B IHC scores of IGF1R, NEDD4, and p-Akt S473 expressions in adjacent non-tumor tissues and primary GC tissues. C Analysis of IGF1 and NEDD4 expressions in normal tissue and GC tissues from Gastric datasets (GSE3468354 and GSE27342, N, normal gastric mucosa, n = 80; T, gastric tumor, n = 80). D Analysis of IGF1R expression in GC stratified by N-cadherin expression and normal tissues, NEDD4 expression in GC stratified by N-cadherin expression and normal tissues, and NEDD4 expression in GC stratified by IGF1 expression and normal tissues in TCGA database. E Protein expression correlation of IGF1R, NEDD4, and p-Akt s473 in GC tissues. F Kaplan–Meier plots of OS among GC patients with different expressions of IGF1, IGF1R, IRS1, and NEDD4. G Overall survival rate of STAD from the TCGA database was analyzed according to the mRNA levels of IGF1 and NEDD4, NEDD4 and N-cadherin. IGF1-High/NEDD4-High ( n = 103); IGF1-High/NEDD4-Low ( n = 82); IGF1-Low/NEDD4-High ( n = 82); IGF1-Low/NEDD4-Low ( n = 103). NEDD4-High/N-cadherin-High ( n = 111); NEDD4-High/N-cadherin-Low ( n = 74); NEDD4-Low/N-cadherin-High ( n = 74); NEDD4-Low/N-cadherin-Low ( n = 111). *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05.

    Article Snippet: The human IRS1-shRNA-1, human IRS1-shRNA-2, human NEDD4-shRNA-1, and human NEDD4-shRNA-2 were contracted into pLKO.1-TRC cloning vector (Addgene, Cambridge, MA, USA; 10878).

    Techniques: Expressing, Immunohistochemistry